Hispanopedia Wiki
Articulo de referencia

Expression cassette

An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell. [ 1 ] In each successful transfo...

Hispanopedia WikiContenido en espanolLectura gratuita

An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell.[1] In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding sequences so that the same cassette can easily be altered to make different proteins.[2]

An expression cassette is composed of one or more genes and the sequences controlling their expression.[3] An expression cassette comprises three components: a promoter sequence, an open reading frame, and a 3' untranslated region that, in eukaryotes, usually contains a polyadenylation site.[4]

Different expression cassettes can be transfected into different organisms including bacteria, yeast, plants, and mammalian cells as long as the correct regulatory sequences are used.[5][6]

See also

References

  1. ↑Papadakis, E.D.; et al. (2004). "Promoters and Control Elements: Designing Expression Cassettes for Gene Therapy". Current Gene Therapy. 4 (4): 89–113. CiteSeerX 10.1.1.494.3472. doi:10.2174/1566523044578077. PMID 15032617.
  2. ↑Bertrand, Edouard; et al. (1997). "The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization". RNA. 3 (1): 75–88. PMC 1369464. PMID 8990401.
  3. ↑Vickers, Claudia E (December 2013). "Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae". Microbial Cell Factories. 12 (96): 96. doi:10.1186/1475-2859-12-96. PMC 4231455. PMID 24161108.
  4. ↑ Gray, Steven James; et al. (enero de 2015). "Elementos de casete de expresión viral para mejorar la especificidad y expresión del objetivo del transgén en la terapia génica" . Discov Med . 19 (102): 49– 57. ISSN 1539-6509 . PMC 4505817. PMID 25636961 .    
  5. ↑ Azhakanandam, K.; et al. (2015). "Diseño de casetes de expresión génica de rasgos vegetales". Avances recientes en la expresión génica y tecnologías facilitadoras en cultivos . Springer. págs. 41–52 . doi : 10.1007/978-1-4939-2202-4_2 . ISBN   978-1-4939-2201-7. S2CID 82188623 . 
  6. ↑ Chow, Yu-Hua; et al. (diciembre de 1997). "Desarrollo de un casete de expresión específico del epitelio con elementos reguladores de ADN humano para la expresión de transgenes en las vías respiratorias pulmonares" ( PDF) . Actas de la Academia Nacional de Ciencias . 94 (26): 14695– 14700. Bibcode : 1997PNAS...9414695C . doi : 10.1073/pnas.94.26.14695 . PMC 25096. PMID 9405675 .